Search results for " recombinant" in Articles / App Notes
Article
Antibody Purification Handbook
The use of recombinant technology opens up the potential to create an infinite number of combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulatin…
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Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
… followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatography of recombinant antibodies expressed transiently in mammalian human embryonic kidney 293T-cells at the…
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Optimization of Protein Expression in Escherichia Coli
There are many parameters that affect cell growth and recombinant protein expression, such as choice of medium, carbon source, temperature, pH, aeration, inducer concentrations, and length of inductio…
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USP Publishes Monoclonal Antibody Guidelines
In 2012, USP started working on a clearly defined set of quality expectations for recombinant therapeutic mAbs, United States Pharmacopeia (USP) General Chapter “Analytical Procedures for Recombinant…
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Comparing Protein A Resins for Monoclonal Antibody Purification
The gene for Protein A has been cloned and expressed in Escherichia coli (6, 7) allowing for the production of large quantities of recombinant Protein A.
Although recombinant Protein A is widely u…
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Bioprocessing Advances in Vaccine Manufacture
…ity (to be operated by G-Con, developer of the modular units being used) in Texas for manufacture of recombinant tobacco plant-expressed influenza vaccines, with a projected final scale capacity of 1…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Capillary electrophoresis sodium dodecyl sulfate nongel sieving analysis of a therapeutic recombinant monoclonal antibody: a biotechnology perspective. Anal Chem. 1999;71:2390–97.
20. Ma S. Analys…
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Quality by design for biotechnology products—part 1
These strategies include engineering the primary sequence to incorporate chemical and post-translational modifications for improved stability; recombinant and chemical fusion approaches for improved h…
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Exploring the world of antibodies: mAbs, Fabs, dAbs, and bsAbs
There is a vast array of antibody variants, including multispecific (msAb), bispecific antibodies (bsAb), antibody fragments (Fab, dAb, scFv), and antibody-drug conjugates (ADC). But with this…
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A deep dive into optimizing AAV capture and polishing to maximize both yield and purity
To meet these needs, Cytiva developed scalable capture and polishing chromatography steps for recombinant AAV (rAAV). The affinity capture step maximizes recovery and efficiently removes impurities.
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