Search results for " purification"
Article
Re-use of Protein A Resin: Fouling and Economics
Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography has remained the de facto capture step for purification of monoclonal antibod…
Article
Next Generation Process Chromatography
One of the main remaining challenges is that each purification cycle today typically takes >3 hours, and when considering ancillary operations, a typical bioprocess designed today will usually run thi…
Article
Optimization of Protein Expression in Escherichia Coli
Many problems are associated with protein expression, including toxicity, misfolding and degradation, aggregation in inclusion bodies, low yields, and difficulties in purification. One of the most com…
Article
Cytiva Advances Fiber Chromatography Technology with New Launch
27, 2021, Cytiva announced the launch of HiScreen Fibro PrismA, the newest product available for early monoclonal antibody (mAb) purification process development. The product complements HiTrap Fibro …
Article
New Therapies Present Scaling Challenges
The downstream purification process can be scaled up, as the purification process for a gene therapy can use similar hardware as is used in mAb purification.
“In general, purification processe…
Article
The Challenge of Disruptive Technologies in Bioprocessing
The biomolecule purification scheme can be very complex, particularly in the case where affinity solutions are not available, Terova continues. Purification can typically include four to five chromato…
Article
Impurity Testing of Biologic Drug Products
“Other host cell proteins and binding agents carried over from purification columns may mimic the action of the therapeutic protein in assays, leading to mis-formulation of the product outside the the…
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Increasing upstream titers can make the Protein A chromatography process step the rate-limiting step in downstream purification. Protein A chromatography columns are frequently too large, limiting the…
Article
Improving Process-Scale Chromatography
By Cynthia Challener, PhD
Chromatography is a crucial step in the purification of biopharmaceuticals and accounts for a significant portion of downstream processing efforts (time and cost) for bio…
Article
Downstream Processing Continues to Worry CMOs
…hese problems, respondents were asked to identify actions they had implemented to improve downstream purification operations.
Among all survey respondents, the most common action taken was to c…