Search results for " DBC"
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How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
In some cases, you might need to determine the DBC yourself to screen for the best resin and running conditions for your specific protein.
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Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The result of process optimization was a DBC of >100 mg mAb/mL medium at a loading condition of pH 5.0 and 50 mM NaCl. A Tricorn 5/100 column (Cytiva) was used to verify the DBC at 10% breakthrough (Q…
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Comparing Protein A Resins for Monoclonal Antibody Purification
According to the vendor, Resin 3 exhibits higher DBC than Resin 2 at longer residence times (8). A residence time of 6 min is expected to give a DBC (at 10% breakthrough) of approximately 60 g antibod…
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Re-use of Protein A Resin: Fouling and Economics
Approximately 80% of industrial respondents have observed a decrease in DBC and flattening of the slope of the breakthrough curve as the cycle number increases, particularly with MabSelect SuRe. For o…
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Determining and Optimizing Dynamic Binding Capacity
While the same general method is used to determine the DBC for many different biomolecules, matrices, and chromatography operations, all of these variables can influence which process parameters are i…
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Get more from UNICORN™ software with plug-in extensions
Access extensions for UNICORN software from the new Cytiva Marketplace, including a free extension that performs Dynamic Binding Capacity (DBC) calculations for breakthrough volume and QB valu…
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Dynamic Binding Capacity in Fiber-Based Chromatography
Thus, determining the DBC of a Fibro unit is still a key step in your process.
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Protein L affinity resin is ready to move to manufacturing
Although protein L affinity resins have been available for more than a decade, insufficient binding capacities and alkaline stability challenges have limited their use in productive manufactur…
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How To Interpret Protein Binding Capacity: Chromatography Resins
The comparison between protein binding capacities of resin from different suppliers is not always straightforward. Different methods may have to be used, and often these methods are not stated direc…
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Biopharmaceutical Process Development: 2018 Lessons and 2019 Predictions
The biopharmaceutical industry continues to expand in new and exciting ways. What might be in store for 2019, and what important lessons should process developers take from 2018? Here, we look back …