Search results for " polishing"
Poster
Purification platform challenges and strategies for handling of "tricky" MAbs
In the work presented here, capture using high-capacity protein A medium (resin) was followed by polishing using a novel cation exchange chromatography medium.
Poster
Three-step monoclonal antibody purification processes using modern chromatography media
This application note describes monoclonal antibody (MAb) purification processes using the expanded mAb purification toolbox of Cytiva business, covering modern chromatography media (resins) for sta…
Article
High-Throughput Process Development in an Historical Environment
Two different novel multimodal polishing Capto™ ImpRes resins were evaluated and the optimal ligand density was identified to provide best aggregate clearance and largest operating space.
In addit…
Article
Challenges of Protein Aggregation During Purification
Hydrophobic interaction chromatography is generally employed as a polishing step for removing impurities based on hydrophobicity. At the same time, strong hydrophobic interactions between the resin an…
Article
Q&As with Industry Leaders
… Northrop, BioPharm International
Jakob Liderfelt, Global Product Manager, Monoclonal Antibody Polishing, Cytiva
Top challenges in recombinant protein purification process development…
Article
Trends in Downstream Bioprocessing
…product purification through affinity chromatography, crystallization, or precipitation; and product polishing and virus removal.
Downstream processing (DSP) has seen significant challenges over …
Article
A Platform Approach for the Purification of Domain Antibodies (Dabs)
This Application note describes a three-step purification process of a domain antibody (Dab) expressed in the periplasm of E. coli. First, a capture step using Capto™ L was used to reduce E. coli host…