Search results for " polishing"
Article
Optimizing KiH bispecific antibody chromatography steps
In addition, two flow-through polishing steps using orthogonal binding principles, hydrophobic interaction and hydrophobic/anion exchange, further reduced the impurities to achieve a monomeric purity …
Webcast
Webinar: Intensifying Downstream Bioprocessing With Continuous Technologies
We will discuss:
-Intensification of capture chromatography and the benefits of coupling with viral inactivation
-How a single flow through chromatography step can simplify polishing
-The rol…
Article
Fundamentals of hydrophobic interaction chromatography
Hydrophobic interaction chromatography (HIC) is a versatile technique that can be used for capture, intermediate purification, or polishing steps.
This technique separates proteins accor…
Article
Optimization of a two-step purification method for bispecific antibodies
Bispecific antibodies (bsAbs) have gained increasing interest over recent years because they can bind two antigens, offering unique modes of action compared to monoclonal antibodies. New and i…
Article
mRNA: The messenger - LNP formulation
Join us for part 5 of mRNA: the messenger, where we discuss key considerations for the formulation of lipid nanoparticles (LNPs) for mRNA drug delivery, including mixing, encapsulation, polishing and …
Article
Capture of Bispecific Antibodies and Removal of Product-Related Impurities
One potential solution is to initiate polishing at the capture step using differences in avidity. In this article, Cytiva presents data on applications using MabSelect™ VL protein L resin with novel …
Article
Top Challenges to Overcome When Developing AAV Processes: Part 1 of 2
… 4:27 – Three main challenges for downstream AAV process development, including cell lysis and the polishing step for full capsid enrichment
7:28 – Challenges associated with full and empty ca…
Poster
Comparison of Three Different Screening Techniques for Predicting Step Elution Conditions for a Cation Exchanger
Screening of chromatographic conditions can be done in several ways, such as 96-well plates, mini-columns, and tips. It is important that the screening can give an indication of the optimal conditio…
Article
Next Generation Bioprocesses: Mapping Roads to the Future
In downstream processing, it can be intensified capture, in-line virus inactivation, flow through polishing, in-line virus filtration, or single-pass filtration.
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Article
Increasing Productivity in Hydrophobic Interaction Chromatography (HIC) Using Capto™ Resins
In this study, the hydrophobicities of newer-generation Capto™ HIC resins were compared to Sepharose™ High Performance and Sepharose Fast Flow HIC resins. An application example is also present…