Search results for " IgG" in Articles / App Notes
Article
Enhancing Resins Addresses Purification Concerns
Christoph Burgstedt/Stock.Adobe.com
Enhancement of resin technology can improve the ability of chromatography to purify the growing landscape of increasingly complex biomolecu…
Article
Development of Purification for Challenging Fc-Fusion Proteins
LMW fragment quantification was performed using a method that involves the heating of IgG samples in the presence of sodium dodecyl sulfate (SDS) under non-reducing conditions. A capillary electrophor…
Article
Fusion Proteins Pose Manufacturability Challenges
“These fusion proteins include an Fc region, are taken from an IgG, that extends the serum half-life of the fusion partner, facilitates expression, and allows purification by protein A affinity chroma…
Article
Improving Process-Scale Chromatography
The new affinity resins work for new antibody types, smaller antibody fragments, and fusion proteins where Protein A chromatography will not because they bind other portions of IgGs. “Affinity resins …
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
Surface plasmon resonance is helping define bispecific antibodies, the next-generation of biopharma therapeutics.
By Robert Karlsson
Abstract
Biotherapeutic antibodies are still the larg…
Article
Re-use of Protein A Resin: Fouling and Economics
Protein A ligand on the resin can undergo nonspecific, noncovalent binding with the IgG and thereby result in detachment from the resin and co-elution with the antibody during elution. Also, dissoluti…
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
Through protein engineering, the amino acids in one of the IgG-binding domains particularly sensitive to alkali were identified and substituted with more stable ones.
A novel prototype Resin 3 off…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
The success of this platform in its various stages has been demonstrated for several MAbs representing different IgG classes produced in different cell lines.
Recent advances in mam…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Of the classes of immunoglobulins, IgG1 is the most commonly used for pharmaceutical and biomedical purposes.2
In recent years, increased understanding of disease and advances in drug discover…
Article
Milestones and Moderate Progress in 2012 Drug Approvals
…ctor (VEGF)-binding portions derived from VEGF receptors 1 and 2, fused to the Fc portion of a human IgG. The resultant 115 kDa dimeric glycoprotein acts as a soluble receptor for human VEGF, thereby…