Search results for " Protein A" in Articles / App Notes
Article
QbD and PAT in Upstream and Downstream Processing
To gain perspective on the implementation of quality by design (QbD) and process analytical technology (PAT) in biopharmaceutical processing, BioPharm International spoke with Clinton Weber, as…
Article
PDA's Technical Report for Biotech Cleaning Validation
The authors encourage biotech manufacturers to consult PDA Technical Report No. 49 for a detailed perspective on current practices and issues in biotech cleaning validation.
By Anurag S. Rathore, D…
Article
Technology Innovations Improve Process Chromatography Performance
Deorkar also notes that there is a lack of diversity in the supply chain of Protein A resin, which is used in the first downstream chromatography step in monoclonal antibody (mAb) manufacturing. “That…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Series S Sensor Chip CM5, Series S Sensor Chip NTA, Series S Sensor Chip Protein A HBS-EP buffer (10 mM Hepes, 0.15 M NaCl, 3 mM ethylene diamine tetra acetic acid [EDTA], and 0.05% [v/v] surfactant P…
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Managing Residual Impurities During Downstream Processing
Challener
Manufacture of protein and other biologic drug substances via cell culture or fermentation occurs with the natural, simultaneous production of other unwanted materials, particularly host-…
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Q&As with Industry Leaders
…sful Development of Scalable AAV Processes: Part 2 of 2
Anki Magnusson, Staff Research Engineer, Protein and Viral Production, Cytiva
Åsa Hagner-McWhirter, PhD, Senior Scientist, Biop…
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The Development and Application of a Monoclonal Antibody Purification Platform
Using this process, the pH of the Protein A affinity chromatography eluate was adjusted upward after pH inactivation and loaded directly onto the anion exchange resin. The anion exchange flow-through …
Article
Milestones and Moderate Progress in 2012 Drug Approvals
First gene therapy and plant-based expression vector products approved in 2012.
In terms of product approvals, the past year can be considered reasonably successful. Twelve biopharm…
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Top Tips for Successful Development of Antibody Chromatography Processes
So let's assume that we have a starting platform, or a process, based on protein A, ion exchange step. So, a cation exchange step and an anion exchange step. If we look at this process, the typical st…
Article
Optimizing your Cell Culture Process to Improve Productivity
We also offer protein quality analysis, including charge variance, glycosylation, and protein aggregation. These types of results provide the scientists information on critical attributes of the prote…