Search results for " Protein A"
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Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Series S Sensor Chip CM5, Series S Sensor Chip NTA, Series S Sensor Chip Protein A HBS-EP buffer (10 mM Hepes, 0.15 M NaCl, 3 mM ethylene diamine tetra acetic acid [EDTA], and 0.05% [v/v] surfactant P…
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Managing Residual Impurities During Downstream Processing
Challener
Manufacture of protein and other biologic drug substances via cell culture or fermentation occurs with the natural, simultaneous production of other unwanted materials, particularly host-…
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Q&As with Industry Leaders
…sful Development of Scalable AAV Processes: Part 2 of 2
Anki Magnusson, Staff Research Engineer, Protein and Viral Production, Cytiva
Åsa Hagner-McWhirter, PhD, Senior Scientist, Biop…
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The Development and Application of a Monoclonal Antibody Purification Platform
Using this process, the pH of the Protein A affinity chromatography eluate was adjusted upward after pH inactivation and loaded directly onto the anion exchange resin. The anion exchange flow-through …
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Milestones and Moderate Progress in 2012 Drug Approvals
First gene therapy and plant-based expression vector products approved in 2012.
In terms of product approvals, the past year can be considered reasonably successful. Twelve biopharm…
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Top Tips for Successful Development of Antibody Chromatography Processes
So let's assume that we have a starting platform, or a process, based on protein A, ion exchange step. So, a cation exchange step and an anion exchange step. If we look at this process, the typical st…
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Optimizing your Cell Culture Process to Improve Productivity
We also offer protein quality analysis, including charge variance, glycosylation, and protein aggregation. These types of results provide the scientists information on critical attributes of the prote…
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Analytical Considerations for Successful Upstream Process Development with Spivey and Lane
Spivey and Lane
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Analytic considerations for upstream processing – covering Process Analytic Technology (PAT), time-savings versus off-line ana…
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Tips for Purifying and Analyzing Cryo-EM Samples
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Cryogenic electron microscopy (cryo-EM) provides possibilities to determine structures for more challenging proteins, such as membrane proteins and larger protein compl…
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Getting a Handle on Biopharma’s Most Critical Quality Attributes and Quality Control
Studies and rankings showed that aggregation and Protein A fragmentation were crucial CQAs, Adkins said. Upstream, the most important CQAs include glucose concentration and cell mobility; downstream, …