Search results for "chromatography" in Articles / App Notes
Article
Combine Chromatography Techniques: Get the Right Purity/Yield of Protein
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Article
Development of Purification for Challenging Fc-Fusion Proteins
…orporates flow-through strategy in polishing steps using cation exchange or hydrophobic interaction chromatography.
As tested on three Fc-fusion proteins, this process demonstrates high …
Article
Evolution of the Monoclonal Antibody Purification Platform
Typical steps include:
• Protein A chromatography for capture of the product and removal of host cell-related impurities (host cell proteins and DNA). Though extremely efficient and effective,…
Article
Enhancing Resins Addresses Purification Concerns
However, precipitation or size-exclusion chromatography (SEC) are not cost-effective when scaled up,” Deorkar says.
Affinity chromatography resins such as oligo(dT) can be…
Article
A Closer Look at Affinity Ligands
Affinity chromatography is a separation method based on the specific binding interaction between an immobilized affinity ligand and its binding partner. The most common example in the realm of bioproc…
Article
Continuous Manufacturing: A Changing Processing Paradigm
They also note that continuous capture typically requires sterile chromatography columns. “For downstream polishing steps, the infancy of multi-column chromatography systems is a limitation,” they add…
Article
Digitalization: The Route to Biopharma 4.0
For chromatography, relating data from sensor with the CQAs of the process has been shown to improve CQA predictions with errors less than 5% (9). In two-column capture chromatography, dynamic control…
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Re-use of Protein A Resin: Fouling and Economics
Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography has remained the de facto capture step for purification of monoclonal antibod…
Article
SEC in the Modern Downstream Purification Process
Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six decades ago, size-exclusion chromatography (SEC) ha…
Article
Trends in Downstream Bioprocessing
It involves the removal of insoluble cell debris and particulates; product isolation through extraction, adsorption, ultrafiltration, or precipitation; product purification through affinity chromatogr…