Search results for " concentration"
Article
Single-Use Workflow for Recovery of a Domain Antibody from E. coli Culture Feed in an Automated Manner
This application note demonstrates the performance of the automated ÄKTA™ readyflux single-use filtration system in microfiltration applications. Here, a domain antibody (dAb) expressed in E. col…
Article
Modeling and Miniaturization: Key Topics at Successful HTPD Conference
This year's clear winner was “Implementation of an Analytical Microfluidic Device for the Quantification of Protein Concentrations in a High-throughput Format” presented by Therese Schermeyer from Kar…
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Although Protein A resins introduced to the market in the last 10 years withstand low to medium concentrations of sodium hydroxide for cleaning, like 0.1 M sodium hydroxide and in some cases 0.5 M, th…
Poster
A Next Generation Protein A Chromatography Resin for Improved Productivity and Bioburden Control
mAb production using a Protein A capture step has followed a highly successful synergistic path the last 30 years. There are, however, remaining challenges.
• The increased upstream titers in mA…
Poster
Impact of sporicidal agent on MabSelect SuRe™ protein A resin lifetime
Bacterial endospore contamination constitutes a challenge in bioproduction due to their resistance to high concentrations of NaOH, commonly used in sanitization procedures. Hence, there is an intere…
Article
Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
Downstream processing goals include multiple stages of purification and concentration. Disposable, single-use filtration is an alternative to outdated centrifugation methods that are difficult to scal…
Article
Single-use Bioreactors Have Reached the Big Time
Newer technologies have more recently been introduced for determination of viable cell mass, glucose, and lactate concentrations.
For instance, Sartorius’ Viamass sensor for online viable cell den…
Poster
Collaborating to address the bioburden challenge
For example, development of Protein A resins to withstand high NaOH concentrations and investigations of an oxidizing agent for sanitization are conducted.
Article
Evaluating E&L Studies for Single-Use Systems
It is important to highlight that in this type of extraction study design, if no dilution is applied from drug substance to drug product, the results in terms of concentration of the detected compound…
Poster
Validation of Cytodex™ Gamma microcarrier-based virus production in single-use bioreactor systems
Cells in each WAVE Bioreactor system were infected with influenza virus and the concentration of infectious virus was determined by 50% tissue culture infective dose measurement. The results show viru…