Search results for " media" in Articles / App Notes
Article
Improving Upstream Predictability
Today, better understanding of cells and how they interact with their environment has enabled improvements in most aspects of upstream processing, from bioreactor design and media development to proce…
Article
An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
Cell-culture applications are impossible without the use of nutrient media for cell multiplication and subsequent product generation. Although several serum-free and chemically defined nutrient media …
Article
Platform Approach Speeds Process Development
Chinese hamster ovary (CHO)-based cell culture platforms should combine state-of the-art technologies for genetic vectors, host cell portfolio, cell line generation, cultivation media, and process des…
Article
Ensuring the Biological Integrity of Raw Materials
Stopping contamination
The most common type of contamination incident happens when a media component is contaminated. For example, bovine serum can be contaminated with reovirus, epizootic hemorrh…
Article
Tools for Continuous Bioprocessing Development
The device can use up to 324 cultures simultaneously, allowing it to be used for process optimization, screening, and media optimization (14, 15).
• Most recently, Sartorius Stedim’s TAP ambr 15 an…
Article
Use of Multivariate Data Analysis in Bioprocessing
Similarly, application of surface-enhanced Raman scattering (SERS) spectroscopy for simple and fast analysis of cell-culture media degradation has been demonstrated (16). In this work, chemometric too…
Article
QbD and PAT in Upstream and Downstream Processing
Timing of events and generation of basal and feed media are controlled with traditional process controls and standard operating procedures. Online cell-density measurement is an area of active develop…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The typical platform process is generally comprised of an initial capture step with Protein A-chromatography, a first polishing step with cation-exchange media, and a final polishing step using anion-…
Article
Re-use of Protein A Resin: Fouling and Economics
The cost of Protein A resin is nearly 50% higher than traditional chromatographic media with nonproteinaceous ligands (2, 10). Resin cost for a large column (>1-m diameter) can exceed $1 million. What…
Article
Cell-Culture Advances Test Bioreactor Performance Models
The continual development of higher-producing cell lines, the switch to chemically defined media, and other advances in cell-culture technology are, however, challenging developers of modeling and sim…