Search results for " media" in Articles / App Notes
Article
A Platform Approach for the Purification of Domain Antibodies (Dabs)
This Application note describes a three-step purification process of a domain antibody (Dab) expressed in the periplasm of E. coli. First, a capture step using Capto™ L was used to reduce E. coli host…
Article
Elucidating Biosimilars Characterization
…ing expression systems and differences regarding the manufacturing processes, be it cell lines used, media used, differences in downstream processing or processing between the innovator and the biosi…
Article
Regulatory Challenges in the QbD Paradigm
…es, if needed, or limited hold steps where appropriate
• Lifetime use studies for purification media (i.e., resins and membranes)
• Validation and verification of cleaning processes, especial…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The possibility to serially connect Capto Q and Capto Core 700 media columns allows for enhanced system utilization and a reduced overall process time.
Before the sterile filtration step, the vi…
Article
PDA's Technical Report for Biotech Cleaning Validation
TOC also measures other sources of organic carbon, including media, cellular materials, detergents, and organic process materials. If total protein is used as a nonspecific analytical method for the a…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
However, because of their large size, their binding capacity is usually very low and chromatographic media with normal pore sizes used for proteins offer no advantages since the pores are not availabl…