Search results for "Recovery"
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Managing Residual Impurities During Downstream Processing
Requirements for system suitability (negative control solution, sensitivity, and linearity), spike recovery of extracted samples, and precision of sample replicates are covered but the limit for resid…
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Mechanistic Modeling of Preparative Ion-Exchange Chromatography
For example, for the commonly used ion-exchange chromatography, pH, ion concentration, gradient, and stationary phase can affect the recovery step as well as the resulting product quality.
For com…
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Single-use Bioreactors Have Reached the Big Time
Sartorius has also introduced a new single-use centrifugation system that allows product recovery from the material removed during the cell bleed step. Currently, this product is typically discarded w…
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Efficient purification of meningococcal polysaccharides in a two-step chromatography workflow
This application note demonstrates the purification of meningococcal capsular polysaccharides using chromatography. The described process constitutes a viable alternative to the traditional proce…
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Efficient Purification of Pneumococcal Polysaccharides in a Chromatography Workflow
… pneumococcal polysaccharides to eliminate the need for phenol extraction, while increasing process recovery and yield.
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Article
Affinity Chromatography Handbook in 3 Volumes
Affinity chromatography (AC) separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The techni…
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Affinity chromatography Handbook - Vol. 3: Specific Groups of Biomolecules
Affinity chromatography (AC) separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The techni…
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Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
This strategy, initially applied to the recovery of cell sheets from t-flask type culture devices (5), has been extended successfully to microcarrier-based cell cultures (3). A key issue in this appro…
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Going Small to Achieve Success on the Commercial Scale
“Demonstration of both individual unit and linked unit output (recovery, product quality attributes, product purity, chromatographic profiles/transition analysis, etc.) comparability between small and…
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Selecting the Right Viral Clearance Technology
…e a purified protein that meets specifications and are developed to maximize purity with reasonable recovery. Only then is a chromatography step tested for viral clearance. “One solution is to add a …