Search results for "downstream" in Articles / App Notes
Article
Sephadex™ Size Exclusion Resin Turns 60
Do you know the story behind Sephadex, which enabled today's large-scale production of biopharmaceuticals? An inadvertent discovery in 1959 became the foundation for today's biopharma industry. This i…
Article
Ion Exchange Chromatography: Introducing New Capto™ HiRes Columns
These new-generation columns are the latest IEX columns delivering the highest resolution, for laboratory-scale purification.
• Designed for the preparation of highly pure proteins and for prote…
Article
Viral Clearance: 7 Chromatography Column Considerations
Viral clearance studies are a critical part of the production of biologics. However, performing studies that are accurate and cost-effective can be challenging.
Here are seven things to conside…
Article
Tips for High Resolution Ion Exchange Chromatography to Obtain a Pure Protein
A high-resolution ion exchange chromatography run is key for obtaining high protein purity. The better the separation between peaks, the higher the purity of the proteins that will be collected.
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Article
Fiber-Based Protein A Chromatography for HTPD
Developing a process or looking for a clone of interest often requires screening large numbers of samples. Many analyses require purified proteins, and often substantial hands-on time is required fo…
Article
Selectivity in Size Exclusion Chromatography (SEC): It’s Much More than Average Pore Diameter (Å)
Describing the pore of a size exclusion chromatography resin with a single number such as average pore diameter is an oversimplification. The formation of pores is dependent on complex combinations of…
Article
Case Study: Virus Reduction of an Affinity Capture Step
Evaluating how much a process step contributes to viral clearance is an essential part of process validation. For this reason, there is an industry need to perform effective viral clearance studies. I…
Article
Fundamentals of Viral Clearance Studies with a Focus on Chromatography
Effective viral clearance studies remain one of the challenges facing manufacturers of biologics. These studies are an essential part of process validation and are critical to ensure drug safety. This…
Article
How To Interpret Protein Binding Capacity: Chromatography Resins
The comparison between protein binding capacities of resin from different suppliers is not always straightforward. Different methods may have to be used, and often these methods are not stated direc…
Article
Reference Guide: Empty Chromatography Columns for Lab Scale
This guide offers an overview of Cytiva's empty chromatography columns designed for use in lab scale, including:
• Main technical specifications
• Compatibility with base matrix
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