Search results for " capture"
Article
Evolution of the Monoclonal Antibody Purification Platform
Typical steps include:
• Protein A chromatography for capture of the product and removal of host cell-related impurities (host cell proteins and DNA). Though extremely efficient and effective,…
Article
Successfully Moving Regulated Data to the Cloud
The document provides clarification on how data should be captured to most effectively preserve its integrity, primarily in the current good manufacturing practice (CGMP) regulated space (1).
The d…
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Digitalization: The Route to Biopharma 4.0
In two-column capture chromatography, dynamic control can be obtained using adaptive model-based control strategy that led to process yield of more than 85%. The data from high performance liquid chro…
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Improving Process-Scale Chromatography
The development of new resin chemistries including enhancements to Protein A for monoclonal antibody (mAb) capture, increasing use of automation, implementation of single-use technologies, and explora…
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A Look at the Affinity Chromatography Landscape
Resin suppliers have in recent years been challenged to increase the productivity of the capture chromatography process to enable more efficient purification of increasingly high titers. They have als…
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Continuous Manufacturing: A Changing Processing Paradigm
They also note that continuous capture typically requires sterile chromatography columns. “For downstream polishing steps, the infancy of multi-column chromatography systems is a limitation,” they add…
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Enhancing Resins Addresses Purification Concerns
Affinity chromatography resins such as oligo(dT) can be used as a capture step, but these resins are not able to separate single-stranded RNA (ssRNA) from double-stranded RNA (dsRNA), and they have a …
Article
The Need for Advanced Process Modeling for New Therapeutic Biologics
For example, the bioprocess model would have to capture relevant information consistently as well as thoroughly to allow both researchers and IT developers understand the process requirements. The mod…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The typical platform process is generally comprised of an initial capture step with Protein A-chromatography, a first polishing step with cation-exchange media, and a final polishing step using anion-…
Article
Innovative Chromatography Resins Can Improve Purity and Quality
A generic capture step for mAbs and Fc fusion proteins has been robustly established by classical Protein A affinity resins, says Oliver Schub, director of Business Development at Navigo Proteins, a s…