Search results for " harvest"
Article
Emerging Therapies Test Existing Bioanalytical Methods
Researchers at the National Institute of Health’s Clinical Center treated two children with adenosine deaminase deficiency using the patient’s own white blood cells that had been harvested, had normal…
Article
Biopharmaceutical Process Development: 2018 Lessons and 2019 Predictions
…pment of downstream chromatography steps for the purification of adenovirus from HEK293 cell culture harvest.
Single-Use Technologies Are Still Growing—But Not Without Challenges
Single-use t…
Article
Developing an Efficient Adenovirus Downstream Purification Process
This application note describes the development of downstream chromatography steps for purification of adenovirus from HEK293 cell culture harvest.
To read the application note click her…
Article
Understanding Validation and Technical Transfer, Part 3
But it is possible to ensure the processing steps work reliably and do not compromise the harvested cells, and that the cell treatment process, whatever it is, has been evaluated and shown to be effec…
Article
Putting Viral Clearance Capabilities to the Test
“Additional routine bulk harvest testing and the viral clearance study complete the tripod of viral safety testing,” she says.
Challenges in study design
The multi-step nature of bioprocesses—…
Article
Cleaning Protein A Resins with 1 M NaOH
The presence of cell culture nutrients in the harvest feed loaded on the capture column also exposes the protein A step to increased risk of microbial contamination. Historically, protein A resins hav…
Article
Development of Purification for Challenging Fc-Fusion Proteins
In most two-column downstream processing platforms, the first column is Protein A (ProA), which binds the target mAb directly from the harvested cell culture fluid. A low pH buffer is routinely used t…
Article
Process Chromatography: Continuous Optimization
Even so, there are many impurities present in the harvest cell-culture fluid obtained after removal of the cells or yeast and media. Host-cell proteins, host-cell DNA, other process-related contaminan…
Article
Hybrid Bioprocessing: A Q&A with Andrew Sinclair
Process Development Forum speaks about hybrid bioprocessing with Andrew Sinclair, President and Founder of BioPharm Services
How would you define hybrid bioprocessing?
Hybrid processing is n…
Article
Single-use Bioreactors Have Reached the Big Time
The company plans to go deep into the technical and process details for media consumption and harvest operations to determine how single-use bioreactors can be optimized based on how these operations …