Search results for " protein"
Article
Purifying Tagged Proteins: Learn the Basics
When and why should you use it? Learn the basics in our quick guide to tagged protein purification.
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What If You Could Remove Your Antibody Aggregates Already in the Protein A Step?
…ies: (1) elution buffer type, (2) conductivity of the elution buffer, (3) pH gradient length and (4) Protein A resin type.
Article
Rapid Cycling Protein A-Based Fiber Chromatography to Address Emerging Downstream Bottlenecks
The protein A fiber matrix has an open pore structure in which mass transfer is governed by convective flow. This structure allows high monoclonal antibody (mAb) binding capacities at very short resid…
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Tips for High Resolution Ion Exchange Chromatography to Obtain a Pure Protein
The better the separation between peaks, the higher the purity of the proteins that will be collected.
Read the full article and get tips and tricks on how to obtain well-separated chromatogram p…
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Single-Step, Protein A Chromatography Process for Bispecific Antibodies in Early Screening
MabSelect™ PrismA protein A resin was selected for the capture step. Most fragments could be removed as early as the capture step using gradient elution from pH 5 to 3.5, without the need for addition…
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Interpreting Protein Binding Capacities for Chromatography Resins
In this post, we explain what protein binding capacity is, how it can be measured, and what to look for on a manufacturer's specification sheet.
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Protein Purification Handbooks
Make sure you have the latest protein purification handbooks!
Whether you are a beginner or an expert, Cytiva handbooks provide practical tips and in-depth information about protein purif…
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Simplify Every Step of Histidine-Tagged Protein Purification
Learn more in this blog post how automated histidine-tagged protein purification can simplify your protein purification.
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Protein Characterization Using SEC: Three Webinar Takeaways
With more than thirty years in protein science, Åke Danielsson, Research Director at Cytiva, recently held a webinar on protein characterization using size exclusion chromatography (SEC). Here, …
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Principles and methods for automated multistep protein purification
Read principles Q&A blog post
Methods of Automated Multistep Protein Purification
Summarized questions related to methods of multistep processing.
Read methods Q&A blog post