Search results for " yeast"
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
Non-animal derived hydrolysates from soy, wheat, and yeast are commonly used in cell culture media.
Protective agents and detergents
These chemicals are typically used to reduce the shear stres…
Article
Process Chromatography: Continuous Optimization
Even so, there are many impurities present in the harvest cell-culture fluid obtained after removal of the cells or yeast and media. Host-cell proteins, host-cell DNA, other process-related contaminan…
Article
The New World of Biopharmaceutical Manufacturing
The number of drugs that are produced by non-mammalian expression systems, such as yeast and microbes, will continue to climb; these will require more highly engineered microbial bioreactors.
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Article
Ensuring the Biological Integrity of Raw Materials
Those include bacteria, yeast, molds, viruses, and sometimes prions.
Mycoplasmas are the smallest of free-living organisms, and are frequent contaminants of mammalian cell cultures. They can alter…
Article
Real Time Continuous Microbiological Monitoring
…n cell, resulting in the real-time detection of both inert particles and biologics such as bacteria, yeasts, and molds. Fluorescent (or fluorophore) dyes (molecules) can absorb light at one frequency…
Article
Safety Drives Innovation in Animal-Component-Free Cell-Culture Media Technology
The earliest examples of successfully eliminating animal-sourced materials relied heavily on substituting complex, undefined alternatives such as vegetable protein hydrolysates or yeast extracts. Thes…
Article
Analysis of Glycosylation in Biosimilars
…wn of Sephadex beads, for example, in the purification process, and high mannose levels may indicate yeast contamination.
With this information in hand, the next steps are to study the glycoprote…
Article
Managing Biomanufacturing Capacity Expectations
…ell culture, 55% used outsourcing for microbial fermentation, 42% used outsourcing for production in yeast, 33% outsourced for production in plant cells, and 33% outsourced capacity for production of…
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Implications of Cell Culture Conditions on Protein Glycosylation
Rapid production of proteins with primitive glycosylation can be achieved using yeast. Insect cells, generally used with a baculovirus vector in transient fashion, are used mostly for R&D and niche pr…
Article
Advanced Technologies Facilitate Scale-up and Technology Transfer
The situation is different for microbial cultures based on Escherichia coli and yeast, for example. These processes have high growth rates and densities, and thus the demand for oxygen is high, making…