Search results for " techniques"
Article
Maximizing the Effectiveness of Upstream Bioprocessing Through the Combination of Lab Informatics and Instrumentation
The increasing popularity of single-use bioreactor systems and continuous processing techniques have brought both new opportunities and challenges to upstream bioprocessing. Together, these trends hav…
Article
Process Chromatography Selection for Downstream Processing Applications
For these molecules, chromatographic techniques such as ion-exchange, hydrophobic interaction chromatography, and multimodal resins are common. Affinity resins like Capto L (antigen-binding fragments …
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Various techniques are used to study structure-function affinity. The function is measured in terms of specificity (affinity), rate and equilibrium constants, as well as thermodynamic properties. Whil…
Article
Challenges in Cell Harvesting Prompt Enhancements
Cell harvesting involves the separation of the cell-culture from the medium and running it through several techniques: centrifugation, microfiltration, depth filtration, and filtration through absolut…
Article
Biopharma Advances Demand Specialized Expertise
This has, in the past, been clearly demonstrated in, for example, the field of mass spectrometry and is currently evolving with techniques involved in biophysical characterization.
BioPharm: What …
Article
Using Quality by Design to Develop Robust Chromatographic Methods
Quality-by-design principles can be used to understand chromatographic measurement system variability.
Sep 2, 2014
By: Melissa Hanna-Brown, Kimber Barnett, Brent Harrington, Tim Graul, Jim…
Article
Predicting Progress in Protein Aggregation
As a result, significant efforts have been invested in understanding the mechanisms behind aggregation and the establishment of predictive models and more advanced analytical techniques, particularly …
Article
Sterilization Trends for Single-Use Consumables
The main techniques include ionizing radiation (gamma-, electron-beam [e-beam]-, X-ray), gas exposure (ethylene oxide [EtO], vaporous hydrogen peroxide [VHP]), and heat/steam.
The first step in es…
Article
QbD Improves Cell-Culture Process Control
By applying advanced sensor systems combined with mathematical modeling techniques, one can gain an enhanced understanding of the processes that produce the biologic product.
In addition, this …
Article
Process Chromatography: Continuous Optimization
Several interaction modes
There are four major chromatographic techniques employed to purify biologic drug substances: affinity, ion-exchange (IEX), mixed- or multi-mode, and hydrophobic-interaction…