Search results for " chromatography" in Articles / App Notes
Article
The Development and Application of a Monoclonal Antibody Purification Platform
In some cases, the broth was concentrated before the first chromatography step because of facility fit issues. Protein A chromatography was used in the initial capture step, where the antibody in cell…
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Scaling Up Novel Therapies
For example, a new collaboration (1) that involves Cytiva, Cobra Biologics, and UK’s Center for Process Innovation, is working on new ways to use fiber-based chromatography to improve AAV scale-up. Ot…
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N-Glycan Analysis of Biotherapeutic Proteins
The intact mass of the different glycoforms of a glycoprotein can be characterized by high-resolution accurate mass-mass spectrometry (HRAM-MS), coupled to reverse-phase liquid chromatography (RP-LC).…
Article
Downstream Processing Continues to Worry CMOs
(Figure courtesy of author)
Chromatography and ultrafiltration
When it comes to specific steps that contribute to downstream-related capacity constraints, chromatography columns continue to be…
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Q&As with Industry Leaders
Jeanne Linke Northrop, BioPharm International
Nora Ketterer, Manager, Modeling Services, Cytiva
Top Tips for Successful Development of Antibody Chromatography Processes
Jeanne L…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Weak partitioning chromatography (WPC) and a high throughput screening (HTS) tools have been essential for development of a robust two-column platform purification process. The benefits of a two-colum…
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Updating Viral Clearance for New Biologic Modalities
Thus, for example, one reduces the HCPs generated by a cell line by manipulating the buffers and chromatography chemistries to separate the product. In such cases, viruses are absent, and their presen…
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Impurity Testing of Biologic Drug Products
Physicochemical methods can be largely based on compendial procedures and require little development, and parameters for platform techniques such as size-exclusion high-performance liquid chromatograp…
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Preclinical Evaluation of Product Related Impurities and Variants
Obtaining the commercial GCSF formulation requires a series of bioprocessing steps including refolding and chromatography. Often, these processing steps result in formation of certain molecular varian…
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Modeling and Miniaturization: Key Topics at Successful HTPD Conference
HTPD was mainly limited to 96-well plates filled with chromatography resins used for resin selection and initial screening of running condition. Since then, HTPD in chromatography applications has bee…