Search results for " PrismA" in Articles / App Notes
Article
Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
The process, performed with MabSelect™ PrismA Protein A chromatography resin, was scaled up 20-fold from a 70 mL HiScale™ 26 column to a 1400 mL AxiChrom™ 100 column. Equivalent mAb recovery and purit…
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Several years ago, we started a development project to design and validate the next-generation protein A resin, MabSelect PrismA, which was just introduced to the market. Now, 1M NaOH can in fact be u…
Article
Optimizing KiH bispecific antibody chromatography steps
…veloped an optimized capture step for two closely related knob-into-hole (KiH) bsAbs using MabSelect PrismA™ protein A resin which reached a purity of 94%. In addition, two flow-through polishing ste…
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Top challenges in recombinant protein purification process development
…Abs, which do have a great affinity solution that can be used in protein A, such as Cytiva MabSelect PrismA. But then there's also a good platform approach that can be used. So after your affinity st…
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Optimization of a two-step purification method for bispecific antibodies
Bispecific antibodies (bsAbs) have gained increasing interest over recent years because they can bind two antigens, offering unique modes of action compared to monoclonal antibodies. New and i…
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Optimization of a Two-Step Purification Method for Bispecific Antibodies
Alligator Bioscience AB develops antibody-based pharmaceuticals for cancer treatment, specializing in mono- and bispecific antibodies for tumor-directed immunotherapies. Alligator and Cytiva devel…
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WEBCAST ON DEMAND: Accelerate mAb Process Development with Fibro Rapid Cycling Chromatography
In this webcast, Cytiva experts discuss how you can speed up process development with HiScreen Fibro™ PrismA rapid cycling chromatography, including topics like lifetime studies and effective evaluati…
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Platform for Purification of VHH-type Antibody Fragments
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Protein A affinity chromatography resins have been used to purify monoclonal antibodies (mAbs) by the bioprocess industry at all scales for a long time. Howev…
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Top Questions on Fibro Chromatography
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Since Fibro PrismA was launched in April 2020, Cytiva has run a number of webinars on rapid cycling protein A-based fiber chromatography. Here, we highlight a…
Article
Next Generation Process Chromatography
Hardick: With HiTrap™ Fibro PrismA now launched for R&D applications, the teams are working hard to ensure this is followed soon by the launch of the GMP units capable of purifying up to 15-kg mAb in …