Search results for "Protein" in Articles / App Notes
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
…l-culture media are essential raw materials that are required for the manufacture of biotherapeutic proteins. Cell-culture media are composed of multiple components, and therefore, it is difficult to…
Article
Tools for Continuous Bioprocessing Development
The repeated deformation of the endoplasmic reticulum can be detrimental to protein glycosylation. Bioreactors with different chamber volumes will have different circulation times, and hence, some ben…
Article
mAbs to Watch in 2016
And there may be many more of these types of proteins on the horizon—as many as 14 are projected to hit the market in 2016, according to data compiled by EvaluatePharma, in collaboration with BioPharm…
Article
SEC in the Modern Downstream Purification Process
…des ago, size-exclusion chromatography (SEC) has played a significant role in the purifiĀcation of proteins, viruses, enzymes, nucleic acids, and other biomolecules. While SEC may have mostly been a…
Article
Improving PAT for Biologics
There has, however, been significant progress in the application of rapid methods for microbial testing and control, as well as the determination of protein concentration, according to Dhanasekharan. …
Article
High-Throughput Process Development in an Historical Environment
In the presented work, instead an automated platform using 96-well plates for parallel screening of inclusion body solubilization and protein refolding conditions was shown. With this approach, 24 sol…
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
…dimers for bsAb’s, and removal of light chain from mAbs during elution when using MabSelect™ VL protein L resin. The video includes results of these experiments and a discussion of how buffer screeni…
Article
Fundamentals of hydrophobic interaction chromatography
This technique separates proteins according to differences in their surface hydrophobicity, and is suitable for the purification of a large range of molecules:
* recombinant proteins and peptides…
Article
New hydrophobic interaction chromatography (HIC) handbook
HIC is often used as a next step to purify proteins in high-salt conditions, for instance after ion exchange chromatography or ammonium sulfate precipitation. The role of this technique in protein pur…
Article
Strategies and Emerging Technologies for mAb Capture
We will discuss opportunities with the introduction of high-capacity protein A resins, how and when multicolumn chromatography is beneficial, and how emerging technologies such as rapid cycling Fibro …