Search results for "Protein" in Articles / App Notes
Article
As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants o…
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Choose your Optimal Wavelength for Chromatography
…your purification is readily available
For customers with a focus on emerging molecules, proteins, peptides and nucleic acid, ÄKTA pure™ chromatography system is flexible and intuitive, an…
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Chromatography for the Diversified Antibody Pipeline
We maintained a good track record of using the protein for capturing the step of bispecific antibodies. It may place more work on the main polishing steps, however, MabSelect may interact with the cla…
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Enhancing Resins Addresses Purification Concerns
What COVID-19 brings to the table
The COVID-19 vaccines comprise several possible and different modalities, such as messenger RNA (mRNA), attenuated viruses, viral vectors, proteins, and virus l…
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Cytiva Advances Fiber Chromatography Technology with New Launch
The product complements HiTrap Fibro PrismA, the company’s new fiber-bases Protein A platform launched for research applications in 2020.
The Fibro technology features an open porous adsorbent mat…
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Updating Viral Clearance for New Biologic Modalities
Because viruses are typically smaller than downstream impurities such as host cell proteins (HCPs) and other extracellular debris, they require meticulous filtration, even in more established processe…
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Western Blot Detection: Is Film or a Digital CCD Imager Better?
It is based on building an antibody:protein complex via specific binding of antibodies to proteins immobilized on a membrane and detecting the bound antibody with one of several detection methods.
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Using Multiple Techniques in Biosimilar Analysis
Proteins are dynamic molecules that do not exist in a single conformation, but as a complex distribution of an ensemble of different conformations. This dynamic conformational landscape will commonly …
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How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
In some cases, you might need to determine the DBC yourself to screen for the best resin and running conditions for your specific protein.
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Host cell proteins were removed using a ReadyToProcess Capto Core 700 chromatography column.
Capto Core 700 chromatography medium (resin) was used in the second chromatography step of the purifica…