Search results for " impurities" in Articles / App Notes
Article
Use of Multivariate Data Analysis in Bioprocessing
Recently, researchers have described an application of MVDA towards development and optimization of a reversed phase (RP)-HPLC method for separation of metaxalone from its hydrolytic impurities (23). …
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
If used correctly, these new analytical methods can reduce analysis and product development time.
ABSTRACT
Monoclonal antibodies represent a significant portion of sales in the biopharma…
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The properties of octylamine ligand, allowing for binding of impurities over a broad pH range, high salt concentrations, and in various buffer compositions, enable the use of Capto Core 700 chromatogr…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Several types of chromatographic resins, including cation exchange, anion exchange, HIC, CHA, and IMAC, were evaluated to remove the less active molecules along with other product and process-related …
Article
Optimizing KiH bispecific antibody chromatography steps
However, due to their increased complexity bsAbs often have higher levels of impurities in comparison to monoclonal antibodies (mAbs). This presents challenges to downstream processing.
This web a…
Article
Introducing the VH3 ligand ELISA kit
Ensuring the safety and efficacy of antibody-based therapeutics is crucial in biotherapeutic manufacturing. Accurate testing for ligand leakage from affinity resins is a key part of this proce…
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Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecif…
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Guide for selecting affinity resins for mAbs, bsAbs, and antibody fragments
Development of antibody-derived molecules are increasing. Today, molecules such as bispecific antibody (bsAb) and antibody fragments such as Fab, scFv, and VHH are seen in labs all over the w…
Article
Get Started: Purification of mAb Variants - eLearning
These molecules show wider variation in binding affinities and are often more challenging to separate from impurities, such as host cell proteins (HCP), host DNA, aggregates, and isoforms.
In this …
Article
A deep dive into optimizing AAV capture and polishing to maximize both yield and purity
It’s critical to maximize both overall yield and removal of impurities, including empty capsids.
To meet these needs, Cytiva developed scalable capture and polishing chromatography steps for recom…