Search results for " separation"
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Process Chromatography Selection for Downstream Processing Applications
Selecting the appropriate separation technology plays a big role in maximizing productivity in any downstream processing application. Some factors companies may consider when selecting a separation te…
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A Look at the Affinity Chromatography Landscape
Specific binding interactions between biologic molecules and immobilized affinity ligands allow for selective separations. With the advent of next-generation biopharmaceuticals, chromatography materia…
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Updating Viral Clearance for New Biologic Modalities
Potential viral separation challenges center around the diversity or variety of potential viral contaminants, he says. First, dependent on the production cell line, there may be a number of viruses wi…
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Using Quality by Design to Develop Robust Chromatographic Methods
A QbD approach to understanding a measurement system such as a chromatographic method involves more than the demonstration of a depth of understanding regarding the choice of chromatographic separatio…
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Optimizing Resin Performance with Disposable Chromatography Solutions
The Importance of resin structure
BioPharm: How important is resin structure to separation success?
Royce and Lundström (Cytiva): Resin structure is crucial to separation success, and there ar…
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Enhancing Resins Addresses Purification Concerns
“There are several purification techniques that are in use, such as precipitation, tangential flow filtration, or chromatographic separations. However, precipitation or size-exclusion chromatography (…
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Challenges in Cell Harvesting Prompt Enhancements
Cell harvesting involves the separation of the cell-culture from the medium and running it through several techniques: centrifugation, microfiltration, depth filtration, and filtration through absolut…
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Implications of Cell Culture Conditions on Protein Glycosylation
Characterization using mass spectrometry
The aforementioned permethylation procedure allows the separation of glycans with similar masses in the non-derivatized state. For example, two glycans—with…
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Analyzing Proteins Using SEC, MALS, and UHPLC
Better separation always results in a better characterization,” he asserts.
Koza and Birdsall also note that SEC is rather insensitive to small changes in apparent MW, and non-specific interaction…
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A Closer Look at Affinity Ligands
Affinity chromatography is a separation method based on the specific binding interaction between an immobilized affinity ligand and its binding partner. The most common example in the realm of bioproc…