Search results for " HIC" in Articles / App Notes
Article
Tackling Analytical Method Development for ADCs
Early development of cytotoxicity assays and chromatographic assays such as HIC, reduced reverse phase high-performance liquid chromatography (RPHPLC), and SEC is therefore important. She adds that ad…
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Evolution of the Monoclonal Antibody Purification Platform
Mixed-mode resins based on ion exchange along with multimodal interactions can offer single-step operation as an alternative to two-step process of ion exchange and HIC. One such example is Capto Adhe…
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Top challenges in recombinant protein purification process development
Another challenge that they face is this slow development time that can take months even, where since they need to use a different combination of methodologies such as HIC and ion exchange, each of th…
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Enhancing Resins Addresses Purification Concerns
“Additionally, these HIC and AEX resins are known to foul when used as a capture step, and alkaline stability of resins should be considered, such as resins based on polymethacrylate (e.g., Bakerbond …
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Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
What is your response to these concerns?
It is true that Protein A affinity chromatography resins have higher cost per liter compared to, for example, ion exchangers or HIC resins. It is also tru…
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Protein Purification Troubleshooting Guide
Includes general tips and specific help for SEC, AC, IEX, and HIC*. Get practical advice on small changes and active choices that can significantly impact your purification results.
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Optimizing KiH bispecific antibody chromatography steps
Bispecific antibodies (bsAbs) are a promising class of therapeutic agents due to their ability to bind to two different types of antigens or two different epitopes on the same antigen. This a…
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Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…
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Chromatography for the Diversified Antibody Pipeline
The HIC resins also demonstrated as good aggregate removers—we have the usual suspects such as aggregates, post end proteins, and tricky wholesome proteins. Polishing may be more challenging and will …
Article
Fundamentals of Hydrophobic Interaction Chromatography
This article explores the fundamentals and applications of hydrophobic interaction chromatography using Capto™ resins to increase productivity.
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