Search results for " fragments" in Articles / App Notes

Article Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecif…

Article Chromatography innovations for the diversified antibody pipeline
MabSelect™ VH3 resins provides efficiency in capture of bispecific antibodies and antibody fragments such as Fab, scFv, and VHH. Discover how we’re supporting the diverse antibody pipelines with chrom…

Article eBook: Purification of Antibody Therapeutics - Approach for Diversified Pipeline
Today, we see multispecific and bispecific antibodies, antibody fragments, and antibody drug conjugates in the development pipeline. Manufacturing platform approaches conveniently used for many tradit…

Article Single-Step, Protein A Chromatography Process for Bispecific Antibodies in Early Screening
The goal was to separate BsAb monomer from fragments such as the individual heavy and light chain pairs while achieving high purity and yield of BsAb. MabSelect™ PrismA protein A resin was selected fo…

Article Selection Guide: Columns and Resins for Antibody Purification and Immunoprecipitation
Polyclonal antibodies, monoclonal antibodies (mAbs), and antibody fragments are usually purified by affinity chromatography. Resins containing an immobilized ligand (e.g., protein A, protein G, or p…

Article Development of Purification for Challenging Fc-Fusion Proteins
Development of Purification for Challenging Fc-Fusion Proteins This study outlines methods for an alternative protein-polishing process for challenging proteins. …

Article Process Chromatography: Continuous Optimization
Most chromatography vendors have developed new affinity resins designed for use with the next-generation biologic products being developed today, including antibody fragments, bispecific antibodies, a…

Article Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
For particular applications that are more related to research and production of cell fragments, freeze/thaw is a traditional method for cell detachment, where cell viability is not of major concern (1…

Article USP Publishes Monoclonal Antibody Guidelines
Although the general chapter is focused on IgG-type mAbs, the principles of the tests included can apply to other related molecules, such as IgM or other isotype antibodies, antibody fragments, and Fc…

Article PDA's Technical Report for Biotech Cleaning Validation
Although degraded fragments of the active ingredient are measured in a cleaning-validation protocol, analytical method validation is typically performed with the bulk active itself because this typica…

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