Search results for " affinity" in Articles / App Notes
Article
Exploring the world of antibodies: mAbs, Fabs, dAbs, and bsAbs
There is a vast array of antibody variants, including multispecific (msAb), bispecific antibodies (bsAb), antibody fragments (Fab, dAb, scFv), and antibody-drug conjugates (ADC). But with this…
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Insights from process developers - top challenges in recombinant protein purification
In this article, we focus on the challenges in purifying recombinant proteins that lack affinity chromatography solutions.
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Chromatography processes for the diversified therapeutic antibody pipeline
The diversity of antibody variants in today’s pipeline presents unique challenges for purification and we see molecules such as bsAbs, Fabs, dAbs, and others. Conventional chromatography protocols, …
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Balancing AAVs Versus LLVs
Knowledge of adeno-associated variant's infection pathway is based on only a few serotypes. The prevailing understanding is that serotypes bind to different, or have differential affiniti…
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Capture of Bispecific Antibodies and Removal of Product-Related Impurities
The biotherapeutics pipeline is becoming increasingly diverse as antibody variants such as bispecifics, conjugates, and fragments move through preclinical stages to commercial manufacturin…
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Chromatography innovations for the diverse antibody pipeline
To support development of new therapeutic possibilities, manufacturing and process development technologies must also evolve. For antibody variants, platform purification protocols for conve…
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Alkaline stability of Fc and VH3 binding in protein A resins
In the development of antibody purification protocols, you need to know your Ab molecule and its possible affinity binding sites for the chromatography resins. In this study, we have looked at…
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Optimizing capture and polishing steps in an rAAV purification process
The chromatography purification described includes affinity capture to maximize recovery with efficient impurity removal.
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Finding the Needle in a High-Dimensional, Information-Rich Data Haystack Can Be Time Consuming
With these tools, scientists can detect and characterize low molecular weight and low affinity compounds binding to their targets.
Improvements in data analysis software have helped to streamline …
Article
Sanitization Opportunities for Protein A Resins
Here, we describe an approach to safely sanitize our new generation bioprocess protein A affinity resin for antibody purification—MabSelect PrismA—with 30 mM PAA combined with NaOH cleaning-in-place r…