Search results for " column" in Articles / App Notes
Article
Trends in Downstream Bioprocessing
Representative comments included:
• Bottleneck at Protein-A chromatography
• Bottlenecks could occur in the next five years
• Clarification before column purification is a bottleneck probl…
Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
Hindrance or help?
Many disposable chromatography columns meet cGMP and industry standards, and also offer reproducibility, scalability, speed, ease-of-use, and operational safety.
However, chr…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Host cell proteins were removed using a ReadyToProcess Capto Core 700 chromatography column.
Capto Core 700 chromatography medium (resin) was used in the second chromatography step of the purifica…
Article
Insider Recommendations for Getting Started with Mechanistic Modeling of Chromatography
If we think of this as being a way of simulating chromatograms with the help of the computer, you take data related to your chromatography system, to your column, and to the separation that you're doi…
Article
Technology Innovations Improve Process Chromatography Performance
The performance reproducibility in column packing, which is essential for ensuring consistent scale-up, can accelerate technology implementation in manufacturing, says Hemanth Kaligotla, chromatograph…
Article
Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
In this work, a multidimensional scale-up (change of both column diameter and bed height) of a mAb capture step, using the ÄKTA pilot 600 chromatography system, is demonstrated. Column volume per…
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
An amount of cell-culture supernatant corresponding to 50-g antibody per liter resin was applied to the column at 6 min. residence time. This was followed by a two-step washing procedure to remove unb…
Article
Instructional Videos for Bioprocess Equipment
Modules also cover column packing and maintenance for pilot and process-scale columns.
Each mediabook contains detailed videos for the equipment and the software interface.
REGISTER HERE TO AC…
Article
Using Digital Twins to Model Process Chromatography
Once a molecule enters the pore system, its behavior follows the same mechanisms in an adsorber bead packed in a filter plate and a production column. To allow for a prediction of the complete process…
Article
Setting Up Bioprocessing Systems for Digital Transformation
Hahn points out that, for instance, pre-packed single-use chromatography columns of the same type may differ in terms of column packing and resin lot capacity. “In practical application, these differe…