Search results for " column"

Article Downstream Processing Continues to Worry CMOs
(Figure courtesy of author) Chromatography and ultrafiltration When it comes to specific steps that contribute to downstream-related capacity constraints, chromatography columns continue to be…

Article Using Quality by Design to Develop Robust Chromatographic Methods
The column screening strategy employed in our laboratories and for the examples discussed here encompasses four stationary phases; two organic solvents; and acidic, neutral, and basic aqueous mobile p…

Article Trends in Downstream Bioprocessing
Representative comments included: • Bottleneck at Protein-A chromatography • Bottlenecks could occur in the next five years • Clarification before column purification is a bottleneck probl…

Article Single-Use for Downstream Chromatography: Benefit or Hindrance?
Hindrance or help? Many disposable chromatography columns meet cGMP and industry standards, and also offer reproducibility, scalability, speed, ease-of-use, and operational safety. However, chr…

Article Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Host cell proteins were removed using a ReadyToProcess Capto Core 700 chromatography column. Capto Core 700 chromatography medium (resin) was used in the second chromatography step of the purifica…

Article Insider Recommendations for Getting Started with Mechanistic Modeling of Chromatography
If we think of this as being a way of simulating chromatograms with the help of the computer, you take data related to your chromatography system, to your column, and to the separation that you're doi…

Article Technology Innovations Improve Process Chromatography Performance
The performance reproducibility in column packing, which is essential for ensuring consistent scale-up, can accelerate technology implementation in manufacturing, says Hemanth Kaligotla, chromatograph…

Article Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
In this work, a multidimensional scale-up (change of both column diameter and bed height) of a mAb capture step, using the ÄKTA pilot 600 chromatography system, is demonstrated. Column volume per…

Article Comparing Protein A Resins for Monoclonal Antibody Purification
An amount of cell-culture supernatant corresponding to 50-g antibody per liter resin was applied to the column at 6 min. residence time. This was followed by a two-step washing procedure to remove unb…

Article Instructional Videos for Bioprocess Equipment
Modules also cover column packing and maintenance for pilot and process-scale columns. Each mediabook contains detailed videos for the equipment and the software interface. REGISTER HERE TO AC…

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