Search results for "concentration"
Article
Will My Buffers Work When Using an Inline Conditioning System?
Buffer preparation can be both time and space consuming and can easily become a challenge in biomanufacturing. In-line preparation of buffers from highly concentrated, single-component stock soluti…
Article
Unifying Continuous Biomanufacturing Operations
Product quality improvements
The known benefits of continuous manufacturing include: reduction in the degradation effects of proteases (9), a smaller footprint, higher cell concentration/higher pr…
Poster
Development of DoE based fed-batch strategies for high-producing CHO cell cultures
Fed-batch culture is commonly employed to maximize cell and product concentrations in upstream mammalian cell culture processes. Typical standard platform processes rely on fixed-volume bolus feedin…
Article
Reconciling Sensor Communication Gaps
Further on the single-use trend, the biggest gap in downstream monitoring and control is the lack of appropriate single-use sensors beyond the standard devices for pH, conductivity, and product concen…
Article
Continuous Processing for the Production of Biopharmaceuticals
The configuration (Figure 1) has the ability to perform refolding at a much higher concentration and offers lower processing time compared with a corresponding batch process (22, 31). The dynamic mixe…
Article
Tools for Continuous Bioprocessing Development
A combination of perfusion flow and cell density control enabled process optimization of various parameters, such as inducer concentration and perfusion flow rate. Cultures were also shown to be stabl…
Article
Best Practices in Qualification of Single-Use Systems
As a result, they are susceptible to attack by organic solvents as well as acids and bases; some, at high concentrations, can even dissolve polymers. Because biomanufacturing may involve processing of…
Article
Fluid Handling in Biopharma Facilities
Industry experts discuss challenges, trends, and innovations in fluid handling.
By Susan Haigney
Contamination prevention in biopharmaceutical manufacturing is crucial, and the proper handling…
Article
Use of Multivariate Data Analysis in Bioprocessing
…ed media, and the authors concluded that significant chemical changes in terms of cysteine/cysteine concentration occur even when media are stored in the dark at 2-8 °C.
A general workflow for bu…
Article
Ligand-Binding Assays and the Determination of Biosimilarity
FDA, in its May 2014 draft guidance on how to show biosimilarity using clinical pharmacological data, indicated that LBAs, concentration and activity assays, and PD assays were important for biosimila…