Search results for "concentration"
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
“Alternative columns, different buffers, pH, or salt concentrations and in some cases even an organic modifier can all improve performance. Better separation always results in a better characterizati…
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Single-Use Technologies Prove Effective for Viral Vector Process Development
… yields than alternative adherent-cell based systems for virus production, while the inline product concentration offers a decrease in operational volumes and delivers a concentrated, clarified bulk …
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Getting a Handle on Biopharma’s Most Critical Quality Attributes and Quality Control
Upstream, the most important CQAs include glucose concentration and cell mobility; downstream, they include turbidity, pH, and conductivity. In addition, virus, microbe, and endotoxin monitoring and c…
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Mechanistic Modeling of Preparative Ion-Exchange Chromatography
For example, for the commonly used ion-exchange chromatography, pH, ion concentration, gradient, and stationary phase can affect the recovery step as well as the resulting product quality.
For com…
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Optimization of Protein Expression in Escherichia Coli
However, this is problematic with the “all-or-nothing” induction characteristics of the T7-based systems, because low inducer concentration leads to a mixed population. This population is typically co…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Some improvements have been made to decrease the development time of IEC, such as the development of a quaternary solvent system to simultaneously adjust the pH and salt concentration to reduce buffer…
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Implementing Inline Conditioning to Advance Process Intensification
Buffer preparation is still a highly manual activity that often requires a lot of resources due to the large number and overall volume of buffers. With inline conditioning buffers can be prep…
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Improving Upstream Predictability
The company is using in-line sensors and Raman spectroscopy to enable precise measurement of viable cell concentration and individual cell metabolites such as glucose and lactate, he explains. Using r…
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Moving PAT from Concept to Reality
…s (size-exclusion chromatography and ultra-high-performance liquid chromatography [UHPLC]); product concentration (HPLC); and protein charge variants (ion exchange chromatography). Numerous at-line a…
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What’s New in Upstream Technologies
Bench-scale automated cell culture system
The scale-X carbo, from Univercells, is a bench-scale automated cell culture system for the expression and concentration of viral drug substance suited fo…