Search results for "antibodies" in Articles / App Notes
Article
A Look at the Affinity Chromatography Landscape
To meet this need, in 2017 the company developed a new Protein A ligand that offers both greater caustic stability (up to 1 molar sodium hydroxide) and increased binding capacity for monoclonal antibo…
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Tackling Analytical Method Development for ADCs
Capillary isoelectric focusing (cIEF), such as imaged cIEF, has been a reasonable substitute, but experience with monoclonal antibodies (mAbs) suggests that there may be differences in the information…
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Advances in Engineering of Protein-Based APIs
Today, monoclonal antibodies (mAbs) have become one of the predominant forms of protein-based biopharmaceuticals, with humanization and fully human monoclonals contributing to their increasing success…
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Get to know: mAb variants - eLearning
Cytiva has published an interactive course that gives an overview of monoclonal antibodies (mAbs) and current variants used and developed in academia and industry.
Key learnings are:
- Structure…
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Emerging Therapies Test Existing Bioanalytical Methods
In addition, many of the viral vectors may react with antibodies already present in animals and humans. To prevent these pre-existing antibodies from interfering with the program, it is recommended to…
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Process Development: What May Lie Ahead in 2018?
Aligning with this news, Process Development Forum readers showed strong interest in an article discussing next-generation antibodies, which are often more potent and more specific than traditional mo…
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As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants o…
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Re-use of Protein A Resin: Fouling and Economics
Its high specificity for the Fc region of antibodies is the primary reason for its dominance as the affinity ligand of choice in numerous immunological and purification applications (1). Specificity o…
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Purification strategies for antibody-derived entities: bispecifics and fragments
Purification platforms used for many monoclonal antibodies (mAbs) need to be adopted for antibody variants to simplify protocols and improve purity and yield. The safety and efficiency requirement of …
Article
Get Started: Purification of mAb Variants - eLearning
The success of monoclonal antibodies (mAbs) as therapeutics and the multi-domain structure of antibodies has made it possible to create antibody variants, such as bispecific antibodies (bsAbs)…