Search results for " binding"
Article
Enhancing Resins Addresses Purification Concerns
…t able to separate single-stranded RNA (ssRNA) from double-stranded RNA (dsRNA), and they have a low binding capacity (1,2). Deorkar notes that hydrophobic interaction chromatography (HIC) and anion …
Article
Defining Critical Quality Attributes for Monoclonal Antibody Therapeutic Products
(B) CDR binding activity assay, showing that oxidized preparations were equipotent to control.
When SAR studies are conducted with legacy programs, the longer history of product development can be…
Article
Antibody Production in Microbial Hosts
Recent developments in aglycosylated antibodies possessing similar antigen-binding affinity and engineered-specific FcγR binding has led to an enormous interest in these products. In this article, the…
Article
Improving Process-Scale Chromatography
…er improving process economy and robustness in mAb manufacturing processes by increasing the dynamic binding capacity and alkali stability of Protein A resins.
For other chromatography steps, the…
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mAbs to Watch in 2016
Other competitors to dupilumab will likely include AstraZeneca’s Xolair (omalizumab), which was approved in the US in 2003 and works by another mechanism as well (by binding to the Ce3 domain of immun…
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Tackling Analytical Method Development for ADCs
The influence of conjugation on antibody binding must also be evaluated, and a wide range of stability studies must be conducted. Fortunately, both improvements in analytical technology and increased …
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Top Tips for Successful Development of Antibody Chromatography Processes
In this interview, Jakob Liderfelt, Product Manager at Cytiva, discusses main challenges faced during chromatography process development for mAbs and mAb variants such as fragments and bispecific a…
Article
Reimagining Affordable Biosimilars
The sensor surfaces have been modified to allow cell-based binding to mimic real-time binding events. Cell-based binding in SPR and BLI takes into account the cell-surface expression of receptors, all…
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Preclinical Evaluation of Product Related Impurities and Variants
This is followed by routine analysis using an array of tests (In vitro binding assays, in vitro potency assessment, pharmacokinetics, pharmacodynamics, toxicity assessment). The data from these studie…
Article
Technology Innovations Improve Process Chromatography Performance
“That dearth is seen especially through the limited dynamic binding capacity needed to process the high-titer cell culture harvest,” he says.
The performance reproducibility in column packing, whi…