Search results for " cell"
Article
Reducing Cross-Contamination Risks in Process Chromatography
In Lundström’s experience, in addition to highly potent compounds, single-use technologies tend to be used more often for vaccine and cell/gene therapy manufacturing due to the natures of these target…
Article
Handbook: Design of Experiments in Protein Production and Purification
In a protein research lab or during process development, a DoE approach can greatly improve the ef ciency in screening for suitable experimental conditions, for example, for cell culture, protein sepa…
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Single-Use Workflow for Recovery of a Domain Antibody from E. coli Culture Feed in an Automated Manner
This application note demonstrates the performance of the automated ÄKTA™ readyflux single-use filtration system in microfiltration applications. Here, a domain antibody (dAb) expressed in E. col…
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Contract Manufacturing Through the Years
Lonza, already a major player in the contract small-molecule API manufacturing business, acquired Celltech and began establishing its position as a leader in contract biologics manufacturing. Cardinal…
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Improvements in Protein A Chromatography
You mention that you sometimes are asked “Why do you still use Protein A?” Why do you?
Protein A chromatography provides high yield while providing up to 3 logs of host cell protein removal and 4…
Article
Sources of Bioburden You Might Have Missed
What better place for a bacterial contamination to grow than in a well-aerated, nutrient-loaded cell culture medium? Downstream operations also have their share of risk. The open handling introduces m…
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High Throughput Protein Purification at a Glance
The project uses antibodies to study the localization of proteins in human tissues and cells. In a blog post site manager Hanna Tegel and research engineers Anne-Sophie Svensson and Annelie Cajander, …
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Technical and Process Economical Aspects of Using Capto™ Q and ReadyToProcess™Adsorber Q in mAb Polishing
Increasing product titers in upstream cell culture processes pose challenges to downstream purification processes. For efficient operations, downstream purification needs to be able to handle high p…
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Biopharma in 2016: Higher Quality Drugs, Less Expensive Manufacturing
…tream Process Intensification
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
A Second Modular Biopharma Facility…
Article
Biosimilars to Drive Modern Manufacturing Approaches
The manufacturer also has to carefully develop analytical methods and the expression system for the construct and host cell. These activities can help refine the manufacturing process, where incorpora…