Search results for " cell"
Article
Perfusion in the 21st Century
Batch processing, in which all raw materials are introduced into the bioreactor at the beginning of the cell-culture process, have largely been replaced with fed-batch systems, in which additional nut…
Article
Emerging Therapies Test Existing Bioanalytical Methods
Specialty assays used to understand the distribution, function, efficacy, and immunogenicity of gene- and cell-therapy drugs—including cell-based neutralizing assays, qPCR, flow cytometry and enzyme-l…
Article
An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
By Mark Plavsic
Use of continuous cell lines in the manufacture of biological therapeutic products, such as vaccines, recombinant proteins, and monoclonal antibodies, is associated with the concom…
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
A typical bioprocess for a mammalian cell line may have 170 process factors. Using this approach, a 170-dimensional space would require 3.3 x 10119 cultures for full characterization, which is not fea…
Article
Analytical Considerations for Successful Upstream Process Development with Spivey and Lane
Topics covered include:
1:47 - Where the industry is headed with real-time analysis
3:17 - Scaling up
4:57 - Cell perfusion
7:16 - Criti…
Article
Cell-Culture Advances Test Bioreactor Performance Models
The continual development of higher-producing cell lines, the switch to chemically defined media, and other advances in cell-culture technology are, however, challenging developers of modeling and sim…
Article
Optimization of Protein Expression in Escherichia Coli
This population is typically comprised of a few cells expressing large amounts of the target of low quality, and the majority of the cells not expressing anything (4).
Codon usage differs among pr…
Article
Single-Domain Antibodies for Brain Targeting
Inappropriate activation of the Fc receptor-expressing cells by antibodies can lead to cytokine release and toxicity. On the other hand, their long circulation half-life results in poor contrast in im…
Article
ADC Development Robust Despite Lackluster Performance
The agent is internalized by the tumor cell after it is bound to the cell by the mAb end of the ADC molecule. Inside the tumor cell, the ADC breaks down into its components, which releases the cytotox…
Article
Witnessing Major Growth in Next-Generation Antibodies
Drug developers are, however, improving linker technologies and the specificity of newer ADC products, such as the targeting of dividing cells vs. non-dividing cells, and Scott Koenig, president, CEO,…