Search results for " antibody" in Articles / App Notes
Article
A Look at the Affinity Chromatography Landscape
They have also needed to develop resin chemistries more suitable for the growing range of protein-based biologics, such as fusion proteins and antibody fragments, and affinity ligands that can separat…
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Tackling Analytical Method Development for ADCs
The influence of conjugation on antibody binding must also be evaluated, and a wide range of stability studies must be conducted. Fortunately, both improvements in analytical technology and increased …
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Advances in Engineering of Protein-Based APIs
These technologies enhance both the speed and success of developing antibody therapeutics,” she says.
Humanization of mAbs is another well-established method to generate therapeutic antibodies w…
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Get to know: mAb variants - eLearning
Key learnings are:
- Structure and binding domains for antibody variants
- Advantages and disadvantages of different antibody fragments
- Design strategies for bispecific antibodies (bsAbs)
…
Article
Emerging Therapies Test Existing Bioanalytical Methods
When considering the development of flow cytometry methods to detect expression of the CAR, optimal antibody and fluorochrome combinations are determined, antibodies are titrated to maximize sensitivi…
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Process Development: What May Lie Ahead in 2018?
Such next-gen antibodies include antibody drug conjugates, engineered antibodies, bispecific antibodies, antibody fragments, antibody-like proteins and biosimilar antibody therapies.
The productio…
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As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants of protein A, may improve purity and increase removal of impurities.
Cytiva…
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Re-use of Protein A Resin: Fouling and Economics
Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography has remained the de facto capture step for purification of monoclonal antibod…
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Purification strategies for antibody-derived entities: bispecifics and fragments
Purification platforms used for many monoclonal antibodies (mAbs) need to be adopted for antibody variants to simplify protocols and improve purity and yield. The safety and efficiency requirement of …
Article
Get Started: Purification of mAb Variants - eLearning
Purification of bsAbs and antibody fragments isn’t as straightforward as for mAbs. These molecules show wider variation in binding affinities and are often more challenging to separate from impurities…