Search results for " size-exclusion" in Articles / App Notes
Article
Impurity Testing of Biologic Drug Products
Physicochemical methods can be largely based on compendial procedures and require little development, and parameters for platform techniques such as size-exclusion high-performance liquid chromatograp…
Article
Preclinical Evaluation of Product Related Impurities and Variants
In this case, reversed-phase high performance liquid chromatography (RP-HPLC) and size-exclusion chromatography (SEC) were used. While the former quantifies levels of the oxidized, f-Met, and reduced…
Article
Development of Purification for Challenging Fc-Fusion Proteins
Measurement of aggregation levels was determined by size-exclusion chromatography (SEC) using ultra performance liquid chromatography (UHPLC) (Waters) with Empower software that used an Acquity UPLC B…
Article
Being Thorough When Transferring Technology
Communication and taking the time to develop the process are key to successful transfer and scale up of biologics.
By Susan Haigney
Editor's note: The following is an extended version of the…
Article
Downstream Processing for Cell-Based Therapies
Assuming the microcarriers are not biodegradable and not part of the final product formulation, the next DSP step is likely to be the separation of cells by size-exclusion techniques. For example, dea…
Article
Improving Process-Scale Chromatography
This approach combines properties of size-exclusion and adsorption chromatography, according to Ljungqvist. She notes that the new resin has been found particularly suitable for vaccine processes; sma…
Article
Process Chromatography Selection for Downstream Processing Applications
Levison (Pall): [Some commonly used separation technologies include] ion-exchange, mixed-mode, hydrophobic interaction, and size-exclusion chromatography.
BioPharm: What are some new separation te…
Article
Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
We report on a new integrated platform for automated harvesting of whole unclarified cell-culture broths, followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatograp…
Article
Host-Cell Protein Measurement and Control
With an additional separation dimension by high pH reversed phase, ion-exchange, or size-exclusion before LC-MS/MS identification, 2D-LC-MS reduces the interference from dominant peptides digested fro…
Article
USP Publishes Monoclonal Antibody Guidelines
The material is an IgG1, 2mg/vial lyophilized presentation, which is used in the size-exclusion chromatography assay (SEC) and the capillary sodium dodecyl sulfate (SDS) electrophoresis (reduced and n…