Search results for " size-exclusion"
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
Let there be light
For submicron aggregates, investigators routinely use size-exclusion chromatography (SEC), which Olcott says separates particles according to hydrodynamic size. Users can add a …
Article
Tackling Analytical Method Development for ADCs
Feb 01, 2015
By Cynthia Challener, PhD
BioPharm International
Volume 28, Issue 2
The targeted therapy possible with antibody-drug conjugates (ADCs) makes them an attractive class of …
Article
SEC in the Modern Downstream Purification Process
Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six decades ago, size-exclusion chromatography (SEC) ha…
Article
Elucidating Biosimilars Characterization
With regard to aggregation analysis, size-exclusion chromatography (SEC), often with multi-angle light-scattering detection, is widely used for the determination of aggregates. Other methods employed …
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Subsequent purification is accomplished by classical column chromatography techniques with size-exclusion chromatography cited most frequently, which is not a desired step for scale-up (41). The chall…